What is the primary role of the buffer in electrophoresis?

What is the primary role of the buffer in electrophoresis?

• A. To increase the temperature of the solution
• B. To set and maintain the pH
• C. To decrease conductivity of the gel
• D. To stabilize the electrophoresis apparatus

Answer: (B)

The primary role of a buffer in electrophoresis is to set and maintain the pH of the solution. Buffers are essential in this context because they help maintain a constant pH during the electrophoresis process. This stability of pH is crucial, as many biological macromolecules, such as proteins and nucleic acids, are sensitive to pH changes, which can affect their charge and conformation. If the pH fluctuates, it might alter the migration rate of these molecules, leading to inaccurate results. Therefore, a buffer system is employed to ensure that the conditions remain optimal for the separation process by keeping the pH constant, which allows for reliable and reproducible outcomes. The other options, while they may relate to different aspects of electrophoresis, do not directly address the primary function of a buffer. For instance, increasing the temperature of the solution is not a role of the buffer, nor does decreasing conductivity directly correspond to the function of buffers. Stabilizing the electrophoresis apparatus is also unrelated to the chemical role of buffers. Thus, maintaining pH is the focal point of the buffer's function in electrophoresis.

The primary role of a buffer in electrophoresis is to set and maintain the pH of the solution. Buffers are essential in this context because they help maintain a constant pH during the electrophoresis process. This stability of pH is crucial, as many biological macromolecules, such as proteins and nucleic acids, are sensitive to pH changes, which can affect their charge and conformation.

If the pH fluctuates, it might alter the migration rate of these molecules, leading to inaccurate results. Therefore, a buffer system is employed to ensure that the conditions remain optimal for the separation process by keeping the pH constant, which allows for reliable and reproducible outcomes.

The other options, while they may relate to different aspects of electrophoresis, do not directly address the primary function of a buffer. For instance, increasing the temperature of the solution is not a role of the buffer, nor does decreasing conductivity directly correspond to the function of buffers. Stabilizing the electrophoresis apparatus is also unrelated to the chemical role of buffers. Thus, maintaining pH is the focal point of the buffer's function in electrophoresis.