Which enzyme allows creatinine to be measured by coupling the creatinine amidohydrolase reaction to the peroxidase reaction?

Which enzyme allows creatinine to be measured by coupling the creatinine amidohydrolase reaction to the peroxidase reaction?

• A. Urease
• B. Alkaline phosphatase
• C. Sarcosine oxidase
• D. Catalase

Answer: (C)

The enzyme that allows for the measurement of creatinine by coupling the creatinine amidohydrolase reaction to the peroxidase reaction is sarcosine oxidase. This enzyme plays a critical role in the biochemical pathway used in creatinine measurement. In the method involving creatinine amidohydrolase, creatinine is converted to sarcosine. Sarcosine oxidase then catalyzes the oxidation of sarcosine to produce hydrogen peroxide. This hydrogen peroxide can be detected through a peroxidase reaction, which often leads to a color change proportional to the amount of creatinine present in the sample, allowing for quantification. Understanding this pathway is crucial, as the accurate measurement of creatinine is important in assessing kidney function. By leveraging the specific actions of sarcosine oxidase, this method effectively enables the clinical measurement of creatinine, illustrating how enzymes can be utilized in diagnostic assays.

The enzyme that allows for the measurement of creatinine by coupling the creatinine amidohydrolase reaction to the peroxidase reaction is sarcosine oxidase. This enzyme plays a critical role in the biochemical pathway used in creatinine measurement.

In the method involving creatinine amidohydrolase, creatinine is converted to sarcosine. Sarcosine oxidase then catalyzes the oxidation of sarcosine to produce hydrogen peroxide. This hydrogen peroxide can be detected through a peroxidase reaction, which often leads to a color change proportional to the amount of creatinine present in the sample, allowing for quantification.

Understanding this pathway is crucial, as the accurate measurement of creatinine is important in assessing kidney function. By leveraging the specific actions of sarcosine oxidase, this method effectively enables the clinical measurement of creatinine, illustrating how enzymes can be utilized in diagnostic assays.